assistant

Intensity projection

CLIJx-Assistant has these intensity projections in Z available:

• Maximum Z Projection
• Minimum Z Projection
• Mean Z Projection
• Mean Z Projection of pixels above a given threshold
• Median Z Projection
• Standard Deviation Z Projection

You can increase contrast in visualisation of your sample by executing background subtraction in advance.

How to do intensity projections

Open your time lapse data set. Start the CLIJx-assistant and follow these steps:

• Your dataset
  • CLIJx-Assistant Starting point
    • Background subtraction
      • Maximum Z Projection

Furthermore, if you want to project in different directions, consider these methods:

• Reslice Left
• Reslice Right
• Reslice Top
• Reslice Bottom
• Transpose XY
• Transpose XZ
• Transpose YZ

Download video [Image data source: Daniela Vorkel, Myers lab, CSBD / MPI CBG]

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